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iScope fluorescence
For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
Product Detail
For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
The excellent optics and mechanical components make the iScope a state-of-the art microscope that is suitable for fluorescence applications in Life Science and Biotechnical fieldsÂ
The iScope fluorescence microscopy can be supplied with:
• 6-position rotating attachment with 100 W mercury vapor light source
• 3-position slider attachment with 100 W mercury vapor light source
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Highlights
- HWF 10x/22 mm eyepieces
- Binocular and trinocular models
- Optional ergonomic tilting head
- Quintuple revolving nosepiece
- E-plan, plan and plan Fluarex IOS corrected objectives
- 187/230 x 140 mm rackless stage with integrated X-Y stage
- Optional ceramic stage
- Diascopic intensity adjustable 3 W NeoLED Köhler illumination
- Epi-fluorescence 100 W HBo mercury vapor
- Blue and Green LED fluorescence
- iCare sensor for energy saving
- State-of-art microscope
- Excellent optics
- Suitable for fluorescence applications
- CSS – Cable Storage System
- Provided with APL (Antimicrobial Protection Layer)
6-positions rotating attachment
With 100 W mercury vapor light sourceÂ
The 6-positions rotating fluorescence attachments are supplied with a standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for excitation in the blue and green light spectrum and a UV protection shieldÂ
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Some models are also available as in vitro diagnostic medical devices class A – (EU) 2017/746
Contact your Euromex dealer
3-positions slider attachment
With 100 W mercury vapor light source
The 3-positions slider fluorescence attachments are supplied with standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for blue and green excitation and a UV protection shieldÂ
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Some models are also available as in vitro diagnostic medical devices class A – (EU) 2017/746
Contact your Euromex dealer
EYEPIECES
Extended EWF 10x/22 mm eyepieces, Ø 30 mm tubes. Eyepieces can be secured to the tubes. The trinocular head of the Plan infinity corrected models (PLi and PLFi) has a light path selector (100:0 / 50:50) and ± 5 diopter adjustments on both tubes.
The trinocular head of the E-Plan infinity corrected models (EPLi) has a fixed built-in light path beamsplitter (50:50) and ± 5 diopter adjustment on the left tube
HEAD
• Binocular and trinocular Siedentopf type heads with 30° inclined tubes. Interpupillary distance from 48 to 76 mmÂ
• A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)Â
• The trinocular head has a 23.2 mm photo tube. This fits all C-mount adapters supplied with Euromex cameras
To order an iScope® with tilting head use reference IS.3158-xxx (Epli/Pli/PLFi)
To order an iScope® with tilting trino head use reference IS.3159-xxx (EPLi/Pli/PLFi)
NOSEPIECE
Revolving quintuple reversed nosepiece on ball bearings
OBJECTIVES
The EPLi and PLi objectives are suitable for Blue and Green standard filter sets and other filter sets in the visible spectrum.Â
The plan Fluarex PLFi â„¢ objectives are suitable for the entire spectrum including Ultra Violet and VioletÂ
E-Plan EPLi IOS | 4x/0.10 | 10x/0.25 | Â | S40x/0.65 | Â | S100x/1.25 oil |
Plan PLi IOS | 4x/0.10 | 10x/0.25 | 20x/0.40* | S40x/0.65 | S60x/0.85* | S100x/1.25 oil |
Plan PLFi IOS | 4x/0.13 | 10x/0.30 | 20x/0.50* | S40x/0.75 | Â | S100x/1.30 oil |
*Â optionalÂ
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput
STAGE
187/230 x 140 mm rackless stage equipped with double slide holder and integrated 79 x 52 mm mechanical stage. The iScope® rackless stage has no protruding parts, enabling more smooth movements and is safer
Optional: 220 x 157 mm ceramic stage with integrated 75 x 50 mm mechanical stage protects with a very tough ceramic surface, against marks and scratches. The surface also ensures stray light not to be reflected from the stage into the objectiveÂ
CONDENSER FOR BRIGHTFIELD
The standard height adjustable Abbe N.A. 1.25 condenser for brightfield with magnification indication.Â
Also accepts sliders with phase annuli suitable for plan phase 10x/20x or S40x/ S100x oil immersion IOS objectives for economical phase contrast. A slider with a simple dark stop for darkfield up to 400x magnification is also available for this condensor
CONDENSER FOR ZERNIKE PHASE CONTRAST
An optional height adjustable Zernike NA. 1.25 phase contrast disc condenser with darkfield stop position for darkfield contrast observation up to 400x magnification and plan phase 10/20/S40x and S100x oil immersion PLPHi IOS objectives
FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel range is approximately 24 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control
ILLUMINATION
3 W adjustable Köhler NeoLED diascopic illumination with internal 100-240 V power supply
The innovative NeoLED design offers larger apertures, allowing the optical system of the iScope microscope to produce images at higher resolutions, very close to the theoretical diffraction limit of the optics. Other benefits of the NeoLED is the low energy consumption, no heating and a long operating lifetime
The Köhler illumination on models with IOS plan and plan phase infinity corrected system provides high contrast and a maximum achievable resolving power of the optics. Also available for other models as an optionÂ
ILLUMINATION FOR FLUORESCENCE
The 6-position rotating and 3-position slider fluorescence attachments are supplied with:
• a 100 W HBO mercury vapor light source for broad powerful light spectrum. Including an 100-240 V external power supply with indication of the life span of the HBO bulb
• Standard fluorescence filters sets forÂ
– blue excitation (EX460- 490 (BP) DM520 EM500 (LP)Â
– green excitation (EX510-550 (BP) DM580 EM570 (LP) and a UV protection shield
The iScope® fluorescence microscopes equipped with a 100 W mercury vapor light source can be supplied with more specific, optimized and application related fluorescence filters sets (see next pages)
STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS
IS.9745 – Blue excitation filter set
EX: 460-490 nm bandpass DM: 500 nm EM: 520 nm longpass
IS.9746 – Green excitation filter set
EX: 510-550 nm bandpass DM: 570 nm EM: 580 nm longpass
IS.9748 –Â UV excitation filter set
EX: 330-385 nm bandpass DM: 400 nm EM: 420 nm longpass
IS.9747 – Violet excitation filter set
EX 400-410 nm bandpass DM 455 nm EM 455 nm longpass
ICARE SENSOR
The unique iCare Sensor is developed to avoid unnecessary loss of energy. The illumination of the microscope automatically switches off shortly after microscopists step away from their position
CSS – CABLE STORAGE SYSTEM
iScope allows users to easily insert the power cable into the back of the instrument, which enables easy storage. The integrated carrying grip at the back of the microscope ensures safe transportation of the microscope
CONFIGURATIONS
The iScope microscope is available in many configurations and can be upgraded with a variety of contrast types
PACKAGE CONTENT
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Models with 100 W mercury vapor light source have a UV protection shield. All packed in polystyrene boxesÂ

Plan PLPH 10x/0.25 phase contrast objective. 0.17 mm cover glass correction. Working distance 12.2 mm

Plan PLPH 20x/0.40 phase contrast objective. 0.17 mm cover glass correction. Working distance 5 mm

Plan PLPH S40x/0.65 phase contrast objective. 0.17 mm cover glass correction. Working distance 0.37 mm

Plan PLPH S100x/1.25 phase contrast objective. 0.17 mm cover glass correction. Working distance 0.13 mm

E-plan EPLi 4x/0.10 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 15.4 mm

E-plan EPLi 10x/0.25 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 5.8 mm

E-plan EPLi S40x/0.65 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.43 mm

E-plan EPLi S100x/1.25 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.13 mm

Plan PLi 2x/0.05 IOS objective for iScope, no cover glass correction. working distance 18.3 mm. Best to be used together with swing-out condenser

Plan PLi 4x/0.10 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 15.4 mm

Plan PLi 10x/0.25 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 10 mm

Plan PLi 20x/0.40 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 5.10 mm

Plan PLi S40x/0.65 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.54 mm

Plan PLi S60x/0.85 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.14 mm

Plan PLi S100x/1.25 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.13 mm

Plan PLi S100x/1.25 IOS infinity corrected objective. 0.17 mm cover glass correction and built-in iris diaphragm. Working distance 0.13 mm

Plan PLi S100x/1.10 IOS water immersion objective. Working distance 0.14 mm. Cover glass 0.17 mm corrected

Plan PLPHi 10x/0.25 phase contrast IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 10.2 mm

Plan PLPHi 20x/0.40 phase contrast IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 5.1 mm

Plan PLPHi S40x/0.65 phase contrast IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.5 mm

Plan PLPHi S100x/1.25 phase contrast IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.13 mm

Plan Fluarex PLFi 4x/0.13 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 16.3 mm

Plan Fluarex PLFi 10x/0.30 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 12.4 mm

Plan Fluarex PLFi 20x/0.50 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 1.5 mm

Plan Fluarex PLFi S40x/0.75 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.35 mm

Plan Fluarex PLFi S100x/1,30 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.13 mm

Phase contrast slider set with 20x and S100x oil PLPHi IOS infinity corrected objectives and annuli for 20x/S100x oil

Polarization set for iScope: simple rotating polarizer for lamp house and fix polarizer mounted under head

Fluorescence filter set EX435 (BP20) DM455 (LP) EM480 (BP30) optimzed for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block (IS.9749)

Fluorescence filter set EX480 (BP30) DM505(LP) EM535 (BP40) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald, GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block (IS.9749)

Fluorescence filter set EX495 (BP20) DM515 (LP) EM540 (BP30) optimized for Alexa Fluor 514â„¢, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Greenâ„¢ 514, Rhodamine 123, Topaz. Needs empty filter block (IS.9749)

Fluorescence filter set EX540 (BP25) DM565 (LP) EM605 (BP55) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block (IS.9749)

Fluorescence filter set EX540 (BP25) DM565 (LP) EM 620 (BP60) optimized for TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block (IS.9749)

Fluorescence filter EX620 (BP50) DM655 (LP) EM 690 (BP50) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649 MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block (IS.9749)

Fluorescence filter set EX420 (BP40) DM455 (LP) EM 535 (BP40) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block (IS.9749)

Fluorescence filter set EX480 (BP30) DM505 (LP) EM 620 (BP60) for Acridine Orange + RNA, Di-8-ANEPPS, non-ratiometric, DiA, Ethidium Bromide, FMâ„¢ 1-43, LysoTracker Yellow HCK-123, Pulsarâ„¢ 650. Needs empty filter block (IS.9749)

Single photo tube attachment with one 23.2 mm tube for IS.5700 ergonomic tilting head of iScope® infinity systems

Köhler attachment

6-positions fluorescence attachment iScope with Blue and Green filter sets included, without objectives

6-positions fluorescence attachment iScope with 2 empty filter blocks without filters and without objectives

3-positions fluorescence attachment iScope with Blue and Green filter sets included, without objectives

3-positions fluorescence attachment iScope with 2 empty filter blocks without filters and without objectives

Filter block with filter set for excitation in Blue spectrum EX460-490 DM520LP EM500LP. Suitable for 6-positions fluorescence attachment

Filter block with filter set for excitation in Green spectrum EX510-550 DM580 EM570LP. Suitable for 6-positions fluorescence attachment

Filter block with filter set for excitation in Violet spectrum EX400-410 DM455 EM455LP. Suitable for 6-positions fluorescence attachment

Filter block with filter set for excitation in UV spectrum EX330-385 DM420 EM400LP. Suitable for 6-positions fluorescence attachment

Filter block with filter set for excitation in Blue spectrum EX460-490 DM520LP EM500LP. Suitable for 3-positions fluorescence attachment

Filter block with filter set for excitation in Green spectrum EX510-550 DM580 EM570LP. Suitable for 3-positions fluorescence attachment

Filter block with filter set for excitation in Violet spectrum EX400-410 DM455 EM455LP. Suitable for 3-positions fluorescence attachment

Filter block with filter set for excitation in UV spectrum EX330-385 DM420 EM400LP. Suitable for 3-positions fluorescence attachment

Filter block with filter set for excitation in Blue spectrum EX460-495 DM505 EM510-560LP. Suitable for 1-positions fluorescence attachment

Filter block with filter set for excitation in Green spectrum EX510-550 DM580 EM510LP. Suitable for 1-positions fluorescence attachment

100-240 Vac/7,5Vdc 1A power supply for single filter block fluorescence attachment
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