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Oxion fluorescence
For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
Product Detail
For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
The Oxion microscopes for fluorescence are equipped with an epi-illumination with a 100 W mercury vapor lamp. The fluorescence attachment have a rail for maximum 4 filter cubes. The FluoLed model is equipped with a 455 nm LED and one filter cube, especially developed for efficient and fast detection of tuberculosis by the Auramine-O fluorochrome. Other FluoLED wavelengths are also available
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Highlights
- Binocular and trinocular models
- HWF 10x/22 mm eyepieces
- Reversed nosepiece for maximum 5 objectives
- Plan, plan fluarex and plan semi apochromatic fluarex ioS objectives
- Integrated mechanical stage
- Coaxial coarse and fine adjustments
- Height adjustable abbe n.a. 1.25 condenser with iris diaphragm and filter holder
- 3 W lED transmitted illumination
- Fluorescence attachment for maximum 4 filter blocks and with 100 W mercury vapor incident illuminati
- Fluorescence attachment with single filter block and 455 nm (Blue), 560 nm (Green) or 365 nm (UV) in
- Ergonomic stand and head
Oxion Fluorescence
EYEPIECES
• HWF 10x/22 mm eyepieces
• All eyepieces can be secured by an Allen screw
HEAD
• Binocular and trinocular Siedentopf type heads are with 30° inclined tubesÂ
• Interpupillary distance from 50 to 75 mm
• Two ± 5 diopter adjustments
• A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)
NOSEPIECE
• Revolving and reversed nosepiece for maximum five objectivesÂ
• The fluorescence models with mercury vapor lamp are equipped with an orange UV protection shieldÂ
OBJECTIVES
• Plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectivesÂ
• Plan Fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.30 oil immersion IOS objectives
• Plan semi apochromatic Fluarex 4x/0.13, 10x/0.30, S40x/0.75 and S100x/1.28 oil immersion IOS objectives
All S40x, S60x and S100x oil immersion objectives are spring loaded. All optics are anti-fungus treated and anti-reflection coated for maximum light throughputÂ
FOCUSING
• Coaxial coarse and fine adjustments, 200 graduations,1 μm per graduation, 200 μm per rotation, total travel range is approximately 28 mm
• Supplied with an adjustable rack stop to prevent damage to sample and objectives
• The coarse adjustments are equipped with friction control
STAGE
• 150 x 140 mm stage with integrated 76 x 50 mm X-Y mechanical stage
• A ceramic stage is also available on request
• Designed for 2 slides
CONDENSER FOR BRIGHTFIELD
• Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm
• Supplied with a white diffusion filter
• Phase contrast rings mounted in slider can be inserted in the condenser for 10x and S40x or 20x and S100x phase contrast objectives
• A darkfield stop in slider for the 10x, 20x and S40x objectives can also be inserted with magnification indication
CONDENSER FOR FLUORESCENCE
The fluorescence attachment is equipped with an integrated condenser with iris diaphragm and with a field diaphragm
ILLUMINATION FOR BRIGHTFIELD AND PHASE CONTRAST
• All models are supplied with a 3 W adjustable NeoLED illumination with built-in 100-240 V power supply
• Köhler illumination with field diaphragmÂ
ILLUMINATION FOR FLUORESCENCE (HBO)
• Fluorescence attachment with 100 W (HBO) mercury vapor incident epi-illumination and rail for maximum four filter blocks
• Two general purpose filter sets are standard supplied with the attachment: AE.3245 (Blue excitation) and AE.3246 (Green excitation)
• Two other general purpose filter sets AE.3246 (UV excitation) and AE.3247 (Ultraviolet excitation) are also available (More specific filter sets are available on request)
ILLUMINATION FOR FLUORESCENCE (LED)
The FluoLED attachment is standard delivered with a 455 nm LED (EX 425-475, DM 480, EM 485 LP filter set) for detection of tuberculosis with Auramine-O fluorochrome. FluoLED attachments with 365 nm LED (EX 340-390, DM 400, EM 420 LP filter set, DAPI), 470 nm LED (EX 450-490, DM 495, EM 500-525 filter set, FITC/GFP/eGFP) and 560 nm LED (EX 540-580, DM 585, EM 608-690 filter set, TexasRed) are also available
STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS
AE.3245 (Blue EX 465-495 nm DM 505 nm EM 515-555 nm)
AE.3246 (Green EX 520-580 nm DM 600 nm EM 605-665 nm)
AE.3247 (Violet EX 400-440 nm DM 455 nm EM 465 nm LP)
AE.3248 (UV EX 366-389 nm DM 415 nm EM 435-485 nm)
CONFIGURATIONS
The Oxion microscope is available in many configurations and can be upgraded with a variety of contrast types.
PACKAGE CONTENT
• Supplied with power cord, dust cover, white filter, user manual and 5 ml immersion oil for models with S100x objective
• All packed in a polystyrene boxÂ

Infinity semi plan SMP S60x/0.65 IOS objective

Plan Fluarex PLFi S100x/1,30 IOS infinity corrected objective. 0.17 mm cover glass correction. Working distance 0.13 mm

Plan semi apochromatic Fluarex PLF S100x /1.28 oil immersion infinity corrected IOS objective Working distance 0.21 mm

Fluorescence block with general purpose filter set for Blue EX475BP40, DM505LP,EM535BP45. Excellent for FITC, eGFP. Compatible for 5-carboxyfluorescein (5-FAM), Alexa fluor 488, BODIP-FL, Calcein, Cy2, Dio (Dio 18(3)), eGFP, FITC, Fluo-4, Fluor-Jade, FluorX, iLyte Fluor 488, Mito tracker Green, Rhodamine 110, Spectrumgreen 110, SYTO-13, YO-PRO-1, YOYO-1

Fluorescence block with general purpose filter set for Green EM560BP55, DM595LP, EM645BP75. Excellent for Texas Red, Alexa 594, 5-Carboxynaphthofluorescein. Other compatible fluorophores: 5-CNF, 5-ROX (carboxy-X-rhodamine),Alexa Fluor 568, Alexa Fluor 594, BOBO-3, BODIPY-TR-X, BO-PRO-3, Calcium Crimson, Cy3.5, HcRed, HiLyte Fluor 594, Lissamine rhodamine B, Mito Tracker Red, mPlum mRaspberry, resorufin, Rhodamine Red, R-phycocyanin, SpectrumRed, Texas Red, X-Rhodaline (XRITC), YO-PRO-3, YOYO-3

Fluorescence block with general purpose filter set for Violet EX440BP21, DM455LP, EM480BP30. Compatible fluorophores: BOBO-1, BOPRO-1, Cerulean, CFP, POPO-1, PO-PRO-1, SpectrumAqua, SYTO45, SYTOX Blue

Fluorescence block with general purpose filter set for UV EX365BP50, DM400LP, EM450BP40. Compatible fluorophores: 4-Methylumbelliverone, 7-amino-4methylcoumarin, 7-Hydrixy-4-methylcoumarin (pH9), Alexa Fluor 350, AMCA, AMCA-X, Calcein Blue, Calcein White, Cascade Blue, DAPI, Hoechst 33342 & 33258, LysoSensor Blue (pH5), Lyso Tracker Red, marina Blue, Spectrum Blue

Stage with heating elements for Oxion series with PID controller, only with new microscopes

Filter block with filter set for excitation in Blue spectrum EX465-495BP DM505LP EM515-555BP for Oxion Inverso inverted microscope

Filter block with filter set for excitation in Green spectrum EX540-580BP DM600LP EM605-665BP for Oxion Inverso inverted microscope

Filter block with filter set for excitation in UV spectrum EX361-389BP DM415LP EM435-485BP for Oxion Inverso inverted microscope

Filter block with filter set for excitation in Violet spectrum EX415-445BP DM455LP EM465-495BP for Oxion Inverso inverted microscope

Empty filter block for filters and dichroic mirror for Oxion Inverso inverted microscope

Filter block rail, three positions for Oxion Inverso inverted microscope for fluorescence with three empty blocks

Standard 23,2 mm tube for 18 mm diameter photo port of Oxion upright (revision 1) microscopes. (This includes an interface to upgrade the existing photo port from a 18 mm to a 31 mm photo port)

Standard 23,2 mm tube for 31 mm diameter photo port of Oxion upright (revision 2) microscopes and for all Oxion Inverso inverted microscopes

Photo port adapter with 0.33x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/3 inch camera with C-mount

Photo port adapter with 0.5x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/2 inch camera with C-mount

Polarizer 360° rotatable for reflected illmination unit

Set of 6 pcs neutral density filters, diameter 25 mm consisting of the following: ND 0.10 @ 80% transmittance, ND 0.30 @ 50% transmittance, ND 0.50 @ 32% transmittance, ND 1.0 @ 10% transmittance, ND 2.0 @ 1% transmittance, ND 3.0 @ 0.1% transmittance

Set of 12 pcs neutral density filters, diameter 25 mm, consisting of 1 each of the following: ND 0.05 @ 90% transmittance, ND 0.10 @ 80% transmittance, ND 0.20 @ 63% transmittance, ND 0.30 @ 50% transmittance, ND 0.40 @ 40% transmittance, ND 0.50 @ 32% transmittance, ND 0.60 @ 25% transmittance, ND 0.70 @ 20% transmittance, ND 0.80 @ 16% transmittance, ND 1.0 @ 10% transmittance, ND 2.0 @ 1% transmittance, ND 3.0 @ 0.1% transmittance

Standard 23.2 mm tube for Oxion (revision 1)

Oxion Ergo Head Add-on (replacing original head)

Micromanipulator for intracytoplasmic sperm injection (ICSI) suitable with the Oxion Inverso inverted microscope
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